What is NASBA?
Nucleic acid sequence based amplification (NASBA) is a method in molecular biology which is used to amplify RNA sequences. NASBA was developed by J Compton in 1991, who defined it as "a primer-dependent technology that can be used for the continuous amplification of nucleic acids in a single mixture at one temperature." Immediately after the invention of NASBA it was used for the rapid diagnosis and quantification of HIV-1 in patient sera. Although RNA can also be amplified by PCR using a reverse transcriptase (in order to synthesize a complementary DNA strand as a template), NASBA's main advantage is that it works at isothermic conditions - usually at a constant temperature of 41°C. NASBA has been introduced into medical diagnostics, where it has been shown to give quicker results than PCR, and it can also be more sensitive. [Source: wikipedia.com]
Detailed reaction overview: www.premierbiosoft.com/tech_notes/NASBA.html
Who uses NASBA?
Who makes instrumentation for NASBA?
Why do I need the NASBA Analysis software?
Although the NASBA reaction is wonderfully adept at qualifying the presence of the target genetic sequence with a simple yes/no threshold, quantifying the data is a little trickier. The Time-To-Positivity (TTP) method is used to determine the initial concentration in the sample, which requires advanced processing techniques to achieve R² > 0.9 models. We offer:
This is close, but I need a customized version for my instrumentation...
Whether you want more automation, integration, or something entirely different, we can help. Contact us to discuss all the possibilities within your reach!